肾母细胞瘤蛋白1(WT1)重组蛋白说明书

Recombinant Wilms Tumor Protein (WT1)

[ PROPERTIES ]
Source: Prokaryotic expression

Host: E.coli

Residues: Leu310~Leu446

Tags: N-terminal His and GST Tag

Subcellular Location: Nucleus, Cytoplasm

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 10.3

Predicted Molecular Mass: 46.5kDa

Accurate Molecular Mass: 47kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure . Gene Sequencing (extract)

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用