应激诱导磷蛋白1抗体-分析方法-资讯-生物在线

应激诱导磷蛋白1抗体

作者:上海雅吉生物科技有限公司 2020-11-06T00:00 (访问量:1005)

 

中文名称 应激诱导磷蛋白1抗体
别    名 Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; NY REN 11 antigen; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521.  
研究领域 细胞生物  免疫学  信号转导  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, )
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 63kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human STIP1:51-150/543 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).

Function:
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).

Subunit:
Forms a complex with HSC70 and HSPCA/HSP-86 and HSPCB/HSP-84. Interacts with PACRG.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Contains 2 STI1 domains.
Contains 9 TPR repeats.

SWISS:
P31948

Gene ID:
10963

Database links:

Entrez Gene: 10963 Human

Entrez Gene: 20867 Mouse

Entrez Gene: 192277 Rat

Omim: 605063 Human

SwissProt: P31948 Human

SwissProt: Q60864 Mouse

SwissProt: O35814 Rat

Unigene: 337295 Human

Unigene: 258633 Mouse

Unigene: 22614 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (bs-9600R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (bs-9600R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (bs-9600R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STIP1) Polyclonal Antibody, Unconjugated (bs-9600R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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