Description

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31. The standard product used in this kit is isolated from plasma.

ScienCells rat Laminin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat Laminin-specific polyclonal antibodies are precoated onto 8 x 12 strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat Laminin in the sample that is captured in the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice.

Reference

1. Haaparanta, T.; Uitto, J.; Ruoslahti, E.; Engvall, E. Molecular cloning of the cDNA encoding human laminin A chain. Matrix 11: 151-160, 1991.
2. Sasaki, M.; Kato, S.; Kohno, K.; Martin, G. R.; Yamada, Y. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc. Nat. Acad. Sci. 84: 935-939, 1987.
3. Nagayoshi, T.; Mattei, M.-G.; Passage, E.; Knowlton, R.; Chu, M.-L.; Uitto, J. Human laminin A chain (LAMA) gene: chromosomal mapping to locus 18p11.3. Genomics 5: 932-935, 1989.
4. Jaye, M.; Modi, W. S.; Ricca, G. A.; Mudd, R.; Chiu, I.-M.; OBrien, S. J.; Drohan, W. N. Isolation of a cDNA clone for the human laminin-B1 chain and its gene localization. Am. J. Hum. Genet. 41: 605-615, 1987.
5. Sasaki, M.; Yamada, Y. The laminin B2 chain has a multidomain structure homologous to the B1 chain. J. Biol. Chem. 262: 17111-17117, 1987.
6. Mattei, M.-G.; Weil, D.; Pribula-Conway, D.; Bernard, M. P.; Passage, E.; Van Cong, N.; Timpl, R.; Chu, M.-L. cDNA cloning, expression and mapping of human laminin B2 gene to chromosome 1q31. Hum. Genet. 79: 235-241, 1988.