pBRIT TAP
(Plasmid #17520)

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PURPOSE

(Empty Backbone)
DEPOSITING LAB

Michael Rudnicki
PUBLICATION

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.
PRICE

$65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pBRIT-LoxP-TAP-puro
(Search Vector Database)
Backbone size (bp)5200
Vector type
Mammalian Expression, Retroviral, Cre/Lox
Selectable markers
Puromycin

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
None
Tag / Fusion Protein
- TAP (C terminal on backbone)

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ cloning siteSee map (not destroyed)
3′ cloning siteSee map (not destroyed)
5′ sequencing primerna

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses
- UBMTA

DEPOSITOR COMMENTS

TAP tag is 6xHis-TEV-3xFLAG (note that two of the FLAG tags are not canonical--the amino acid sequence of the 3xFLAG region is: DYKDHDGDYKDHDIDYKDDDDK)

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pBRIT TAP was a gift from Michael Rudnicki (Addgene plasmid # 17520)
For your References section:

Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84.10.1038/ncb1671 PubMed 18066051

pBRIT TAP逆病毒载体质粒图谱序列抗性

pBRIT TAP (Plasmid #17520)