Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Gibco® Sf-900™ II SFM features:

• Superior long-term, high density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior Long-Term, High Density Growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells ⁄ mL, a significant improvement over competitors formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20-100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia