Dulbeccos Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium

for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12

include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies

 offers a variety of Gibco DMEM / F-12 modifications for a range of cell culture applications. Find the

 right formulation using the media selector tool.

The complete  formulation is available.

Gibco DMEM / F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high

concentrations of glucose, amino acids and vitamins with F-12更看更多s wide variety of components.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the

intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York.

The facility is registered with the FDA as a medical device manufacturer and is certified to ISO

13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical

Gibco DMEM / F-12 product made in our Scotland facility (32500-043). This facility is registered

with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require

supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate

 buffer system (2.438 g / L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment,

and filtration at the time of preparation (see protocol for details).

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for

Research Use only in non-clinical unit, product information in Chinese shall prevail.)