RAF1 Peptide
产品名称: RAF1 Peptide
英文名称: RAF1 Peptide
产品编号: P2839
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T269 of human RAF1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Role of Raf in vascular protection from distinct apoptotic stimuli.
Alavi A, Hood JD, Frausto R, Stupack DG, Cheresh DA.Science. 2003 Jul 4;301(5629):94-6.
- 2.
- Regulation of Raf through phosphorylation and N terminus-C terminus interaction.
Chong H, Guan KL.J Biol Chem. 2003 Sep 19;278(38):36269-76. Epub 2003 Jul 14.
- 3.
- Down-regulation of Raf-1 kinase is associated with paclitaxel resistance in human breast cancer MCF-7/Adr cells.
Lee M, Koh WS, Han SS.Cancer Lett. 2003 Apr 10;193(1):57-64.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MCF-7 cell lysate (35 ug/lane) with RAF1 polyclonal antibody (Cat # PAB3496).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RAF1 polyclonal antibody (Cat # PAB3496) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5894
- Protein Accession#:
- P04049
- Gene Name:
- RAF1
- Gene Alias:
- CRAF,NS5,Raf-1,c-Raf
- Gene Description:
- v-raf-1 murine leukemia viral oncogene homolog 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
- Other Designations:
- Oncogene RAF1,raf proto-oncogene serine/threonine protein kinase
- Interactome
- Gene Pathway
- Acute myeloid leukemia
- B cell receptor signaling pathway
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Melanoma
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- T cell receptor signaling pathway
- Vascular smooth muscle contraction
- VEGF signaling pathway
- Related Disease
- Abnormalities, Multiple
- Arrhythmias, Cardiac
- Articulation Disorders
- Cardiovascular Diseases
- Cognition
- Cognition Disorders
- Developmental Disabilities
- Diabetes Mellitus, Type 2
- Dyslexia, Acquired
- Edema
- Genetic Predisposition to Disease
- Glioma
- Hearing
- Heart Defects, Congenital
- Hypertrophy, Left Ventricular
- Language Disorders
- LEOPARD Syndrome
- Memory
- Motor Skills