IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including
macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory
cytokines such as IL-1 and TNF-α. Like IL-10, IL-10 enhances humoral immune responses and attenuates cellmediated
immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human
cells.
The BioGems Human IL-10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase
immunoassay specially designed to measure Human IL-10 with a 96-well strip plate that is pre-coated with
mouse monoclonal antibody specific for IL-10. It contains E. coli-expressed recombinant Human IL-10. The
detection antibody is a biotinylated goat polyclonal antibody specific for IL-10. The kit is analytically validated
with ready to use reagents.