Description

Transforming growthFactor-β1 (TGF-β1) is a multifunctional peptide that controls proliferation, differentiation, and otherFunctions in many cell types. Many cells synthesize TGF-β and essentially all of them have specific receptorsFor this peptide. TGF-β regulates the actions of many other peptide growthFactors and can have a positive or negative regulatory affect.1 TGF-β1 is knownFor its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGF-β1 is also an important mediator of bone remodeling. TGF-β1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growthFactors at the site of inflammation. TGF-β1, a multifunctional cytokine withFibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-β1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGF-β1 is secreted as a latentForm, which consists of its matureForm and a latency-associated peptide (β1-LAP) in either the presence or the absence of additional latent TGF-β1-binding protein. The standard product used in this kit is recombinant TGF-β1 with a molecular mass of 25kDa.

ScienCells mouse TGF-β1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse TGF-β1-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse TGF-β1 in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growthFactorβ: biologicalFunction and chemical structure. Science 233: 532-534, 1986.
2. Blanchette,F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGFβ-1 regulates gene expression of its own converting enzymeFurin. J. Clin. Invest. 99: 1974-1983, 1997.
3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van Maldergem, L.; Vanhoenacker,F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the latency-associated peptide of TGFβ-1 cause Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000.
4. Li, A. G.; Wang, D.;Feng, X.-H.; Wang, X.-J. Latent TGF?-1 overexpression in keratinocytes results in a severe psoriasis-like skin disorder. EMBO J. 23: 1770-1781, 2004.
5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGFβ-1 DNA polymorphisms, protein levels, and blood pressure. Hypertension 33: 271-275, 1999.
6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific mutations of a transforming growthFactor (TGF)β 1 latency-associated peptide cause Camurati-Engelmann disease because of theFormation of a constitutively activeForm of TGFβ 1. J. Biol. Chem. 276: 11469-11472, 2001. 

 

 

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       近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。 
     
       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。 确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）： 
       
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