供应DAPI溶液（1mg/ml）
保存：-20℃，有效期至少 2 年 
产品说明： 
DAPI(4’,6-二脒基-2-苯基吲哚二盐酸盐)是一种能够与 DNA 中大部分 A，T 碱基相互结合的荧光染料o常用于荧光显微镜观测。因为 DAPI 可以透过完整的细胞膜o它可以用于活细胞和固定细胞的染色。当 DAPI 与双链 DNA 结合时， 大吸收波长为 358nm， 大发射波长为 461nm。DAPI 的发射光为蓝色，DAPI 和绿色荧光蛋白 GFP 或 Texas Red 染剂(红色荧光染剂)的发射波长仅有少部分重叠，可以利用这项特性在单一的样品上进行多重荧光染色。 
本产品为 DAPI 水溶液，纯度≥90%，浓度为 1mg/ml。使用时根据实验不同直接将本产品用相应溶液稀释到工作浓度。  
使用方法： （仅供参考） 
对于培养细胞 
1， 取适量 DAPI 水溶液加到 PBS 中，制备成 5-15 μg/ml 的 DAPI 溶液。 
2， 将 1/10 培养基体积的 DAPI 溶液加入到细胞培养基中。 
3， 在 37℃培养细胞 10-20 分钟。 
4， 用 PBS 或合适的缓冲液洗细胞两次。 
5， 置于荧光显微镜下观察，激发波长 360-400nm。 
对于组织切片 
制好的玻片上滴加几滴稀释的 DAPI 染液，染色 10 分钟，流水冲去染液，滤纸吸除多余水分，加一滴荧光封片液，置于荧光显微镜下观察。 
注意事项： 
 DAPI 被普遍认为具有致癌性，操作时应戴手套，并避免交叉污染。 
  本产品需避光，并尽量避免反复冻融。 
相关产品： 
  C0080  碘化丙锭 PI溶液（1mg/ml） 
 CA1020 ANNEXIN V-FITC/PI凋亡检测试剂盒 
 12100 DMEM(H)培养基 
 S9000  特级胎牛血清 
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图片为DAPI固体，溶液试剂以实物为准。本产品由索莱宝实验室自己配制，以下为相关试剂
              
         
             
货号
             
试剂名称
             
包装规格
             
价格
         
         
             
R8070
             
剥离硅烷
             
200ml
             
160
         
         
             
B8150
             
亲和硅烷
             
25ml
             
200
         
         
             
C0020
             
Hoechst 33258染色液（即用型）
             
10ml/50ml 
             
120/320
         
         
             
C0030
             
Hoechst 33342染色液（即用型）
             
10ml/50ml 
             
120/320
         
         
             
C0040
             
台盼蓝染色液(0.4%)
             
50ml/100
             
120/200
         
         
             
C0060
             
DAPI溶液（1mg/ml）
             
1ml/1ml*10
             
200/1200
         
         
             
C0065
             
DAPI溶液（即用型）
             
10ml/50ml 
             
120/320
         
         
             
C0080
             
碘化丙锭PI溶液（1mg/ml）
             
1ml/1ml*10
             
80/500
         
         
             
C1010
             
柠檬酸钠缓冲液0.01mol/L， pH6.0，干粉
             
1L/2L
             
8/12
         
         
             
C1013
             
柠檬酸钠缓冲液0.1mol/L， pH4.5，无菌溶液
             
100/500ml
             
50/160
         
         
             
C1031
             
柠檬酸钠抗原修复液（1×）
             
100/500ml
             
50/160
         
         
             
C1032
             
柠檬酸钠抗原修复液（50×）
             
100/500ml
             
70/220
         
         
             
C1033
             
EDTA抗原修复液（1×）
             
100/500ml
             
50/160
         
         
             
C1034
             
EDTA抗原修复液（50×）
             
100/500ml
             
70/220
         
         
             
C1035
             
冰冻切片抗原修复液（1×）
             
100/500ml
             
100/320
         
         
             
C1036
             
细胞爬片抗原修复液（1×）
             
100/500ml
             
100/320
         
         
             
C1050
             
ELISA包被液（1x）
             
100/500ml
             
70/200
         
         
             
C1055
             
ELISA包被液（10x）
             
100/500ml
             
100/300
         
         
             
C1058
             
ELISA终止液
             
100/500ml
             
70/200
         
     
 
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