pBC KS-表达载体质粒
产品名称： pBC KS-表达载体质粒
英文名称： pBC KS-表达载体质粒
产品编号： BiovectorPBCKS-
产品价格： 0
产品产地： Biovector NTCC Inc. USA
品牌商标： Biovector,NTCC, Addgene, ATCC,
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Product Data Sheet
|
Order ID |
Name |
Description |
|
BiovectorPBCKS- |
pBC KS- |
pBC KS-, AmpR.500uL,in E.coli. Storage:4℃ |
Recovery
- Obtain an LB agar plate with the appropriate antibiotic.
- Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
- Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
- Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
- Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
- Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
- In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
|
Source/Vendor: |
Stratagene |
|
Plasmid Type: |
Bacterial Expression |
|
Cloning Method: |
Unknown |
|
Size: |
3400 |
|
5 Sequencing 1 Primer: |
T7 Fwd |
|
Bacterial Resistance: |
Ampicillin |
|
Notes: |
The KS designation indicates the polylinker is oriented such that beta-galactosidase (lacZ) transcription proceeds through the KpnI site first and the SacI site last. |
|
Catalog Number: |
212217, 212218 |
|
GenBank: |
X52329 |
|
Stable: |
Unspecified |
|
Constitutive: |
Unspecified |
|
Viral/Non-Viral: |
Nonviral |
>sequence 3400bp
|
1 |
CTGACGCGCC |
CTGTAGCGGC |
GCATTAAGCG |
CGGCGGGTGT |
GGTGGTTACG |
50 |
|
51 |
CGCAGCGTGA |
CCGCTACACT |
TGCCAGCGCC |
CTAGCGCCCG |
CTCCTTTCGC |
100 |
|
101 |
TTTCTTCCCT |
TCCTTTCTCG |
CCACGTTCGC |
CGGCTTTCCC |
CGTCAAGCTC |
150 |
|
151 |
TAAATCGGGG |
GCTCCCTTTA |
GGGTTCCGAT |
TTAGTGCTTT |
ACGGCACCTC |
200 |
|
201 |
GACCCCAAAA |
AACTTGATTA |
GGGTGATGGT |
TCACGTAGTG |
GGCCATCGCC |
250 |
|
251 |
CTGATAGACG |
GTTTTTCGCC |
CTTTGACGTT |
GGAGTCCACG |
TTCTTTAATA |
300 |
|
301 |
GTGGACTCTT |
GTTCCAAACT |
GGAACAACAC |
TCAACCCTAT |
CTCGGTCTAT |
350 |
|
351 |
TCTTTTGATT |
TATAAGGGAT |
TTTGCCGATT |
TCGGCCTATT |
GGTTAAAAAA |
400 |
|
401 |
TGAGCTGATT |
TAACAAAAAT |
TTAACGCGAA |
TTTTAACAAA |
ATATTAACGC |
450 |
|
451 |
TTACAATTTC |
CATTCGCCAT |
TCAGGCTGCG |
CAACTGTTGG |
GAAGGGCGAT |
500 |
|
501 |
CGGTGCGGGC |
CTCTTCGCTA |
TTACGCCAGC |
TGGCGAAAGG |
GGGATGTGCT |
550 |
|
551 |
GCAAGGCGAT |
TAAGTTGGGT |
AACGCCAGGG |
TTTTCCCAGT |
CACGACGTTG |
600 |
|
601 |
TAAAACGACG |
GCCAGTGAGC |
GCGCGTAATA |
CGACTCACTA |
TAGGGCGAAT |
650 |
|
651 |
TGGAGCTCCA |
CCGCGGTGGC |
GGCCGCTCTA |
TUNEology 波长可调检测卡盒--> |