INDO polyclonal antibody
产品名称： INDO polyclonal antibody
英文名称： INDO polyclonal antibody
产品编号： PAB2571
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of INDO.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human INDO.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemsitry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.
Chauhan N, Basran J, Efimov I, Svistunenko DA, Seward HE, Moody PC, Raven EL.Biochemistry. 2008 Apr 22;47(16):4761-9. Epub 2008 Mar 28.
- 2.
- Cytochrome b5, not superoxide anion radical, is a major reductant of indoleamine 2,3-dioxygenase in human cells.
Maghzal GJ, Thomas SR, Hunt NH, Stocker R.J Biol Chem. 2008 May 2;283(18):12014-25. Epub 2008 Feb 25.
- 3.
- Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
Scheler M, Wenzel J, Tuting T, Takikawa O, Bieber T, von Bubnoff D.Am J Pathol. 2007 Dec;171(6):1936-43. Epub 2007 Nov 30.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of INDO polyclonal antibody (Cat # PAB2571) in 293 cell line lysates (35 ug/lane). INDO (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinomareacted with INDO polyclonal antibody (Cat # PAB2571), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 3620
- Protein Accession#:
- NP_002155;P14902
- Gene Name:
- IDO1
- Gene Alias:
- CD107B,IDO,INDO
- Gene Description:
- indoleamine 2,3-dioxygenase 1
- Omim ID:
- 147435
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM
- Other Designations:
- indole 2,3-dioxygenase,indoleamine-pyrrole 2,3 dioxygenase
- Gene Pathway
- Related Disease