Akt (pan) (40D4) Mouse mAb-蛋白质/抗原/多肽-试剂-生物在线
杭州沃森生物技术有限公司
Akt (pan) (40D4) Mouse mAb

Akt (pan) (40D4) Mouse mAb

商家询价

产品名称： Akt (pan) (40D4) Mouse mAb

英文名称： Akt (pan) (40D4) Mouse mAb

产品编号： #2920

产品价格： 0

产品产地： 美国

品牌商标： CST

更新时间： 2023-09-19T21:32:30

使用范围： null

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Akt (pan) (40D4) Mouse mAb #2920

PhosphoSitePlus protein, site, and accession data: Akt1, Akt2, Akt3
No. Size  
2920S 100 ul ( 20 western blots )  
2920 carrier free & custom formulation / quantity  
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human, Mouse, Rat, Monkey Endogenous 60 Mouse IgG1
IP 1:50
IHC-P 1:250
IF-IC 1:50
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

Akt (pan) (40D4) Mouse mAb detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide at the carboxy-terminal sequence of human Akt.

Western Blotting

Western Blotting

Western blot analysis of recombinant Akt1, Akt2, Akt3 and GST proteins using Akt (pan) (40D4) Mouse mAb (upper) and GST (91G1) Rabbit mAb #2625 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, C6 and COS cells using Akt (pan) (40D4) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Akt (pan) (40D4) Mouse mAb in the presence of control peptide (left) or Akt (pan) Blocking Peptide #1085 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Akt (pan) (40D4) Mouse mAb on SignalSlide Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (pan) (40D4) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, either LY294002-treated (left) or insulin-treated (right), using pan-Akt (40D4) MmAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).


 

Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

  1. Franke, T.F. et al. (1997) Cell 88, 435-7.
  2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
  3. Franke, T.F. et al. (1995) Cell 81, 727-36.
  4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  6. Jacinto, E. et al. (2006) Cell 127, 125-37.
  7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
  8. Brunet, A. et al. (1999) Cell 96, 857-68.
  9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
  10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
  12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
  13. Cross, D.A. et al. (1995) Nature 378, 785-9.
  14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
  16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
  17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

Application References

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