Citric Acid Assay Kit
产品名称: Citric Acid Assay Kit
英文名称: Citric Acid Assay Kit
产品编号: K-CITR
产品价格: 0
产品产地: 爱尔兰
品牌商标: Megazyme
更新时间: null
使用范围: null
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- 邮编 : 200030
- 所在区域 : 上海
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柠檬酸检测试剂盒是一种灵活简单的方法,用于快速可靠地测量和分析食品,饮料和其他材料中的柠檬酸(柠檬酸盐)。
UV-method for the determination of Citric Acid in foods,
beverages and other materials
Principle:
(citrate lyase)
(1) Citrate → oxaloacetate + acetate
(L-malate dehydrogenase)
(2) Oxaloacetate + NADH + H+ → L-malate + NAD+
(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → D-lactate + NAD+
Kit size: * 72 assays (manual) / 720 (microplate)
/ 840 (auto-analyser)* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.491 mg/L
Application examples:
Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products
(e.g. cheese), meat, processed meat, vegetable and fruit products,
bakery products, paper, pharmaceuticals, cosmetics and other
materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by MEBAK, OIV,
EU, ISO2963, AOAC and IFU22
(Note: If the enzyme oxaloacetate decarboxylase is present in the sample, some
of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric
acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed
to efficiently convert any pyruvate produced into D-lactate and NAD+).Advantages
- Reconstituted citrate lyase stable for 4 weeks at 4°C / 6 months at -20°C
- Buffer / cofactor / enzyme tablets for efficient use of kit components
- PVP incorporated to prevent tannin inhibition
- Very competitive price (cost per test)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual, microplate and auto-analyser formats