HHV8gp59 polyclonal antibody
产品名称： HHV8gp59 polyclonal antibody
英文名称： HHV8gp59 polyclonal antibody
产品编号： PAB10254
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of HHV8gp59.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of human herpesvirus 8 type M HHV8gp59.
- Host:
- Rabbit
- Reactivity:
- Viruses
- Specificity:
- A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:100000-1:170000)
Western Blot (1:5000-1:8000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Targeted disruption of Kaposi's sarcoma-associated herpesvirus ORF57 in the viral genome is detrimental for the expression of ORF59, K8alpha, and K8.1 and the production of infectious virus.
Majerciak V, Pripuzova N, McCoy JP, Gao SJ, Zheng ZM.J Virol. 2007 Feb;81(3):1062-71. Epub 2006 Nov 15.
- 2.
- Structural and functional analyses of Kaposi sarcoma-associated herpesvirus ORF57 nuclear localization signals in living cells.
Majerciak V, Yamanegi K, Nie SH, Zheng ZM.J Biol Chem. 2006 Sep 22;281(38):28365-78. Epub 2006 Jul 6.
- 3.
- The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor.
Malik P, Blackbourn DJ, Clements JB.J Biol Chem. 2004 Jul 30;279(31):33001-11. Epub 2004 May 20.
- Applications
- Western Blot (Transfected lysate)
- Western blot using HHV8gp59 polyclonal antibody (Cat # PAB10254) to detect HHV8gp59 in HEK293 cells transfected with HHV8gp59 expression vector and HHV8gp59 truncations, or in HHV8gp59 infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow).
The membrane was probed with the primary antibody diluted 1 : 7,500 (left) and 1:1,000 (right).
Personal Communication, V. Majerciak, M.Zheng, CCR-NC , Bethesda, MD.