D-果糖和D-葡萄糖测定试剂盒
产品名称: D-果糖和D-葡萄糖测定试剂盒
英文名称: The D-Fructose and D-Glucose Assay Kit
产品编号: K-FRGLMQ
产品价格: 0
产品产地: 爱尔兰
品牌商标: Megazyme
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 上海市徐汇区蒲汇塘路11号博大商务楼1307室
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 138****4547 点击查看
- 传真 : 点击查看
- 邮箱 : 3bio@163.com
使用MegaQuant比色计(在505 nm测量),D-果糖和D-葡萄糖MegaQuant格式测试试剂盒适用于测量和分析葡萄,葡萄汁和葡萄酒中的D-果糖和D-葡萄糖。
Simple colourimetric method for the determination of
D-Fructose and D-Glucose in foodstuffs, beverages and
other materials
Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP
(phosphoglucose isomerase)
(3) F-6-P ↔ G-6-P
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(diaphorase)
(5) INT + NADPH + H+ → NADP+ + INT-formazan
Kit size: 60 assays
Method: Spectrophotometric at 505 nm
Reaction time: ~ 5 min
Detection limit: 23.2 mg/L
Application examples:
Grape juice / must, wine, beer, fruit juices, soft drinks, milk, jam, honey,
dietetic foods, bread, bakery products, candies, desserts, confectionery,
ice-cream, fruit and vegetables, condiments, tobacco, cosmetics,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel methodAdvantages
- Novel product, patented technology
- Spectrophotometer / laboratory expertise not required
- Highly stable reagents (at least three seasons use)
- Very competitive price (cost per test)
- Very simple procedure
- Rapid reaction time (~ 10 min)
- Standard included