Origin:
Normal Bronchial Smooth Muscle 
Species:
Homo sapiens 
Tissue:
Bronchus 
Morphology:
Smooth muscle 
Properties:
Adherent 
Medium:
SMC medium + supplement (please refer to Specification sheet) 
Subculture:
1:2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C 
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO 
Biosafety Level:

I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative


References for HBSMC cells:

1. Goto, K., Chiba, Y., Sakai, H., and Misawa, M. Tumor necrosis factor-alpha (TNF-alpha) induces upregulation of RhoA via NF-kappaB activation in cultured human bronchial smooth muscle cells (HBSMC). J Pharmacol Sci, 110: 437-444, 2009. 

2. Kitamura, N., Kaminuma, O., Ohtomo, T., Kiyokawa, N., Kobayashi, N., Suko, M., and Mori, A. Evaluation of cysteinyl leukotriene-induced contraction of human cultured bronchial smooth muscle cells (HBSMC). Int Arch Allergy Immunol, 149 Suppl 1: 83-86, 2009. 

3. Goto, K., Chiba, Y., and Misawa, M. IL-13 induces translocation of NF-kappaB in cultured human bronchial smooth muscle cells (HBSMC). Cytokine, 46: 96-99, 2009. 

4. Suzuki, Y., Asano, K., Niimi, K., Miyata, J., Shiraishi, Y., Fukunaga, K., Shiomi, T., Nakajima, T., Oguma, T., Sayama, K., and Ishizaka, A. TP receptor-mediated release of eosinophil chemotactic activity from human bronchial smooth muscle cells (HBSMC). Eur J Pharmacol, 600: 133-139, 2008. 

5. Kitamura, N., Kaminuma, O., Kobayashi, N., and Mori, A. A contraction assay system using established human bronchial smooth muscle cells (HBSMC). Int Arch Allergy Immunol, 146 Suppl 1: 36-39, 2008. 

6. Nakajima, T., Jo, T., Meguro, K., Oonuma, H., Ma, J., Kubota, N., Imuta, H., Takano, H., Iida, H., Nagase, T., and Nagata, T. Effect of dexamethasone on voltage-gated Na+ channel in cultured human bronchial smooth muscle cells (HBSMC). Life Sci, 82: 1210-1215, 2008.