pMSCV-IRES-GFP II (pMIG II)
(Plasmid #52107)

PURPOSE

(Empty Backbone) a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
DEPOSITING LAB

Dario Vignali
PUBLICATION

Holst et al Nat Protoc. 2006;1(1):406-17.
PRICE

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
MSCV IRES GFP
(Search Vector Database)
Modifications to backbone
expanded MCS
Vector type
Mammalian Expression, Retroviral
Tag / Fusion Protein
- IRES-EGFP (C terminal on backbone)

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Stbl3
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

Partial (1)
Addgene Sequences

Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ sequencing primerpLXSN_5_primer

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials

DEPOSITOR COMMENTS

The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pMSCV-IRES-GFP II (pMIG II) was a gift from Dario Vignali (Addgene plasmid # 52107)
For your References section:

Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263

pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性

pMSCV-IRES-GFP II (pMIG II) (Plasmid #52107)