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iTaq universal SYBR Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq universal SYBR Green supermix lets you:

    Obtain reliable and reproducible gene expression analysis results
    Amplify genomic DNA and difficult amplicons with superior efficiency
    Achieve consistent results across real-time instrument platforms under fast or standard conditions

iTaq universal SYBR Green supermix contains:

    Advanced buffer formulation with SYBR Green I for optimal qPCR performance
    Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
    Blend of passive reference dyes for universal real-time instrument compatibility

Applications and Uses

    Gene expression analysis
    Genotyping
    Mutation detection
    Pathogen detection