Product Information

LOCUS       Z32697                  5721 bp    DNA     linear   SYN 14-NOV-2006

DEFINITION  Expression vector (PAN52-1N) DNA, 5721bp.

ACCESSION   Z32697

VERSION     Z32697.1  GI:475161

KEYWORDS    gpdA gene.

SOURCE      synthetic construct

  ORGANISM  synthetic construct

            other sequences; artificial sequences.

REFERENCE   1  (bases 2305 to 3071)

  AUTHORS   Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and

            Timberlake,W.E.

  TITLE     Primary structure of the trpC gene from Aspergillus nidulans

  JOURNAL   Mol. Gen. Genet. 199 (1), 37-45 (1985)

   PUBMED   3158796

REFERENCE   2  (bases 999 to 2572)

  AUTHORS   Punt,P.J., Dingemanse,M.A., Kuyvenhoven,A., Soede,R.D.,

            Pouwels,P.H. and van den Hondel,C.A.

  TITLE     Functional elements in the promoter region of the Aspergillus

            nidulans gpdA gene encoding glyceraldehyde-3-phosphate

            dehydrogenase

  JOURNAL   Gene 93 (1), 101-109 (1990)

   PUBMED   2121607

REFERENCE   3

  AUTHORS   Punt,P.J., Kuijvenhoven,A., Broekhuijsen,M.P. and van den

            Hondel,C.A.M.J.J.

  TITLE     Vectors for efficient production of homologous and heterologous

            proteins in filamentous fungi

  JOURNAL   Unpublished

  REMARK    (sites)

REFERENCE   4  (bases 1 to 5721)

  AUTHORS   Punt,P.

  TITLE     Direct Submission

  JOURNAL   Submitted (22-APR-1994) Punt P., TNO Nutrition and Food Research

            Institute, Lange Kleiweg 139, Rijkswik, The Netherlands

FEATURES             Location/Qualifiers

     source          1..5721

                     /organism="synthetic construct"

                     /mol_type="other DNA"

                     /db_xref="taxon:32630"

     misc_feature    1..5721

                     /product="fungal expression vector pAN52-1"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /citation=[3]

     gene            1..2457

                     /gene="gpdA"

     promoter        1..2129

                     /gene="gpdA"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /citation=[2]

                     /citation=[3]

                     /function="promoter region of the A.nidulans gpdA gene"

     misc_feature    2130

                     /gene="gpdA"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /function="major transcription start site"

     intron          2178..2293

                     /gene="gpdA"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /citation=[2]

                     /number=1

     CDS             2302..2457

                     /gene="gpdA"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /note="GPDA"

                     /citation=[2]

                     /citation=[3]

                     /codon_start=1

                     /transl_table=11

                     /protein_id="CAA83646.1"

                     /db_xref="GI:475162"

                     /translation="MDPLNVTEIIKQLDESGYKIVGVDVSSGVETNGVQDLDKIRSFV

                     QAAKSAF"

     terminator      2305..3071

                     /standard_name="Terminator region from A.nidulans trpC

                     gene (containing cds)"

                     /experiment="experimental evidence, no additional details

                     recorded"

                     /note="this region contains the 3end of the trpC

                     transcript including 50 codons. two major transcription

                     ends have been identified"

                     /citation=[1]

                     /citation=[3]

     polyA_site      2555

     misc_difference 2565..2575

                     /note="introduction of one base at this position generates

                     an BLGI site with was shown to be present by restriction

                     digestion"

                     /citation=[1]

                     /replace="gccttncaggc"

     misc_difference 2774..2782

                     /note="introduction of one base at this position generates

                     an ALWNI site which was shown to be present by restriction

                     digestion"

                     /citation=[1]

                     /replace="cagcncctg"

     polyA_site      2828

     misc_difference 2862..2872

                     /note="introduction (actual sequence could be deletion as

                     well) of a base at this position results in the

                     elimination of an XMNI site which was shown to be absent

                     by restriction digestion"

                     /citation=[1]

                     /replace="gaaatcanttc"

     variation       2899..2906

                     /note="A NCOI site present at this position was removed by

                     digestion, treatment with klenow polymerase and

                     religation"

                     /citation=[3]

                     /replace="catgcatg"

     misc_feature    3072..5721

                     /product="PUC18"

                     /note="Vector sequences derived from PUC18 from SALI to

                     ECORI"

                     /citation=[3]

>swquence  5721bp

GAATTCCCTTGTATCTCTACACACAGGCTCAAATCAATAAGAAGAACGGTTCGTCTTTTTCGTTTATATC

TTGCATCGTCCCAAAGCTATTGGCGGGATATTCTGTTTGCAGTTGGCTGACTTGAAGTAATCTCTGCAGA

TCTTTCGACACTGAAATACGTCGAGCCTGCTCCGCTTGGAAGCGGCGAGGAGCCTCGTCCTGTCACAACT

ACCAACATGGAGTACGATAAGGGCCAGTTCCGCCAGCTCATTAAGAGCCAGTTCATGGGCGTTGGCATGA

TGGCCGTCATGCATCTGTACTTCAAGTACACCAACGCTCTTCTGATCCAGTCGATCATCCGCTGAAGGCG

CTTTCGAATCTGGTTAAGATCCACGTCTTCGGGAAGCCAGCGACTGGTGACCTCCAGCGTCCCTTTAAGG

CTGCCAACAGCTTTCTCAGCCAGGGCCAGCCCAAGACCGACAAGGCCTCCCTCCAGAACGCCGAGAAGAA

CTGGAGGGGTGGTGTCAAGGAGGAGTAAGCTCCTTATTGAAGTCGGAGGACGGAGCGGTGTCAAGAGGAT

ATTCTTCGACTCTGTATTATAGATAAGATGATGAGGAATTGGAGGTAGCATAGCTTCATTTGGATTTGCT

TTCCAGGCTGAGACTCTAGCTTGGAGCATAGAGGGTCCTTTGGCTTTCAATATTCTCAAGTATCTCGAGT

TTGAACTTATTCCCTGTGAACCTTTTATTCACCAATGAGCATTGGAATGAACATGAATCTGAGGACTGCA

ATCGCCATGAGGTTTTCGAAATACATCCGGATGTCGAAGGCTTGGGGCACCTGCGTTGGTTGAATTTAGA

ACGTGGCACTATTGATCATCCGATAGCTCTGCAAAGGGCGTTGCACAATGCAAGTCAAACGTTGCTAGCA

GTTCCAGGTGGAATGTTATGATGAGCATTGTATTAAATCAGGAGATATAGCATGATCTCTAGTTAGCTCA

CCACAAAAGTCAGACGGCGTAACCAAAAGTCACACAACACAAGCTGTAAGGATTTCGGCACGGCTACGGA

AGACGGAGAAGCCACCTTCAGTGGACTCGAGTACCATTTAATTCTATTTGTGTTTGATCGAGACCTAATA

CAGCCCCTACAACGACCATCAAAGTCGTATAGCTACCAGTGAGGAAGTGGACTCAAATCGACTTCAGCAA

CATCTCCTGGATAAACTTTAAGCCTAAACTATACAGAATAAGATAGGTGGAGAGCTTATACCGAGCTCCC

AAATCTGTCCAGATCATGGTTGACCGGTGCCTGGATCTTCCTATAGAATCATCCTTATTCGTTGACCTAG

CTGATTCTGGAGTGACCCAGAGGGTCATGACTTGAGCCTAAAATCCGCCGCCTCCACCATTTGTAGAAAA

ATGTGACGAACTCGTGAGCTCTGTACAGTGACCGGTGACTCTTTCTGGCATGCGGAGAGACGGACGGACG

CAGAGAGAAGGGCTGAGTAATAAGCCACTGGCCAGACAGCTCTGGCGGCTCTGAGGTGCAGTGGATGATT

ATTAATCCGGGACCGGCCGCCCCTCCGCCCCGAAGTGGAAAGGCTGGTGTGCCCCTCGTTGACCAAGAAT

CTATTGCATCATCGGAGAATATGGAGCTTCATCGAATCACCGGCAGTAAGCGAAGGAGAATGTGAAGCCA

GGGGTGTATAGCCGTCGGCGAAATAGCATGCCATTAACCTAGGTACAGAAGTCCAATTGCTTCCGATCTG

GTAAAAGATTCACGAGATAGTACCTTCTCCGAAGTAGGTAGAGCGAGTACCCGGCGCGTAAGCTCCCTAA

TTGGCCCATCCGGCATCTGTAGGGCGTCCAAATATCGTGCCTCTCCTGCTTTGCCCGGTGTATGAAACCG

GAAAGGCCGCTCAGGAGCTGGCCAGCGGCGCAGACCGGGAACACAAGCTGGCAGTCGACCCATCCGGTGC

TCTGCACTCGACCTGCTGAGGTCCCTCAGTCCCTGGTAGGCAGCTTTGCCCCGTCTGTCCGCCCGGTGTG

TCGGCGGGGTTGACAAGGTCGTTGCGTCAGTCCAACATTTGTTGCCATATTTTCCTGCTCTCCCCACCAG

CTGCTCTTTTCTTTTCTCTTTCTTTTCCCATCTTCAGTATATTCATCTTCCCATCCAAGAACCTTTATTT

CCCCTAAGTAAGTACTTTGCTACATCCATACTCCATCCTTCCCATCCCTTATTCCTTTGAACCTTTCAGT

TCGAGCTTTCCCACTTCATCGCAGCTTGACTAACAGCTACCCCGCTTGAGCAGACATCACCATGGATCCA

CTTAACGTTACTGAAATCATCAAACAGCTTGACGAATCTGGATATAAGATCGTTGGTGTCGATGTCAGCT

CCGGAGTTGAGACAAATGGTGTTCAGGATCTCGATAAGATACGTTCATTTGTCCAAGCAGCAAAGAGTGC

CTTCTAGTGATTTAATAGCTCCATGTCAACAAGAATAAAACGCGTTTTCGGGTTTACCTCTTCCAGATAC

AGCTCATCTGCAATGCATTAATGCATTGACTGCAACCTAGTAACGCCTTNCAGGCTCCGGCGAAGAGAAG

AATAGCTTAGCAGAGCTATTTTCATTTTCGGGAGACGAGATCAAGCAGATCAACGGTCGTCAAGAGACCT

ACGAGACTGAGGAATCCGCTCTTGGCTCCACGCGACTATATATTTGTCTCTAATTGTACTTTGACATGCT

CCTCTTCTTTACTCTGATAGCTTGACTATGAAAATTCCGTCACCAGCNCCTGGGTTCGCAAAGATAATTG

CATGTTTCTTCCTTGAACTCTCAAGCCTACAGGACACACATTCATCGTAGGTATAAACCTCGAAATCANT

TCCTACTAAGATGGTATACAATAGTAACCATGCATGGTTGCCTAGTGAATGCTCCGTAACACCCAATACG

CCGGCCGAAACTTTTTTACAACTCTCCTATGAGTCGTTTACCCAGAATGCACAGGTACACTTGTTTAGAG

GTAATCCTTCTTTCTAGAAGTCCTCGTGTACTGTGTAAGCGCCCACTCCACATCTCCACTCGACCTGCAG