The RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE is for the extraction of total nucleic acid from formalin-

or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up

 to four 20 µm sections, or up to 35 mg of unsectioned, core samples each. Features of the RecoverAll™ Total Nucleic

Acid Isolation Kit for FFPE:

• Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue
• No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon
• Typical yields are >50% of those of unfixed tissue
• Recovered nucleic acids are suitable for real-time RT-PCR, PCR, mutation screening, and microarray analyses

Extraction of nucleic acids from difficult samples
Archived tissue samples contain valuable information of disease states, but it has traditionally been difficult to

 isolate nucleic acids from them of a quality suitable for molecular analysis. 更看更多Standard preservation techniques

use formalin that maintains tissue structure and prevents putrefaction, but which also traps nucleic acids and modifies

 them through protein-protein and protein-nucleic acid crosslinks. RNA (and to some extent DNA) is often so fragmented

and chemically modified that it is incompatible with many molecular analysis techniques. RNA fragmentation in FFPE tissues

cannot be reversed; however, the protease digestion conditions of the RecoverAll™ kit are designed to release a maximal

amount (see figure) of trapped RNA fragments of all sizes, including microRNA, in a relatively short amount of time.