KLF10 polyclonal antibody
产品名称： KLF10 polyclonal antibody
英文名称： KLF10 polyclonal antibody
产品编号： PAB3463
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of KLF10.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human KLF10.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Estrogen receptor beta isoform-specific induction of transforming growth factor beta-inducible early gene-1 in human osteoblast cells: an essential role for the activation function 1 domain.
Hawse JR, Subramaniam M, Monroe DG, Hemmingsen AH, Ingle JN, Khosla S, Oursler MJ, Spelsberg TC.Mol Endocrinol. 2008 Jul;22(7):1579-95. Epub 2008 May 15.
- 2.
- Two novel VHL targets, TGFBI (BIGH3) and its transactivator KLF10, are up-regulated in renal clear cell carcinoma and other tumors.
Ivanov SV, Ivanova AV, Salnikow K, Timofeeva O, Subramaniam M, Lerman MI.Biochem Biophys Res Commun. 2008 Jun 13;370(4):536-40. Epub 2008 Mar 24.
- 3.
- TIEG1 induces apoptosis through mitochondrial apoptotic pathway and promotes apoptosis induced by homoharringtonine and velcade.
Jin W, Di G, Li J, Chen Y, Li W, Wu J, Cheng T, Yao M, Shao Z.FEBS Lett. 2007 Aug 7;581(20):3826-32. Epub 2007 Jul 16.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of KLF10 polyclonal antibody (Cat # PAB3463) pre-incubated with and without blocking peptide in CEM cell line lysate. KLF10 (N-term) (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human prostate carcinomareacted with KLF10 polyclonal antibody (Cat # PAB3463), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.