M-MLV逆转录酶
产品名称： M-MLV逆转录酶
英文名称： M-MLV Reverse Transcriptase
产品编号： 28025013
产品价格： 0
产品产地： 中国美国
品牌商标： Invitrogen
更新时间： 2023-09-19T20:36
使用范围： null
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- 地址 : 杭州市西溪路525号浙江大学科技园B座501室
- 邮编 : 310029
- 所在区域 : 浙江
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- 邮箱 : service@ws-bio.com
| 产品名称 | 货号 | 规格 | 目录价（RMB） |
| M-MLV Reverse Transcriptase | 28025013 | 2000 | |
| 40,000 units | |||
| M-MLV Reverse Transcriptase | 28025021 | 9440 | |
| 200,000 units |
描述
M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase
that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
Thermostability – Optimal activity at 37°C
Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3),
cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease,
and ribonuclease assays; and yield and length of cDNA product.
详细说明
| Optimal Reaction Temperature: |
37° C |
| Final Product Size(s): |
7 kb or less |
| Ribonuclease H Activity: |
Reduced |
| Concentration: |
200 U?μl |
| Reverse Transcriptase: |
M-MLV Reverse Transcriptase |
| Form: |
Frozen |
| Format: |
Tube(s) |
| Template: |
ssRNA, RNA-DNA Hybrid, ssDNA |
| Sensitivity: |
Low |
| Product Size: |
40,000 units |
| Enzyme Function: |
RNA dependent DNA Polymerase |
| Shipping Condition: |
Wet Ice |
| Number of Reactions: |
200 Reactions |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures |
内容和储存
M-MLV RT is supplied with a vial of 5X first strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM
KCl, 15 mM MgCl2 ], and a vial of 100 mM DTT. Store at -20°C. Guaranteed stable for 6 months when properly stored