Sulfo-EMCS交联剂(水溶性)

中文名称：Sulfo-EMCS交联剂(水溶性)
英文名称：Sulfo EMCS
产品规格：50mg
CAS号：103848-61-9
EINECS号：999-999-2
分子量：308.3
储存条件：冷冻（-20℃）

概述

EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide
(NHS)ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.NHS
esters react with primary amines at pH 7-9 to form amide bonds,while maleimides react with sulfhydryl groups at pH
6.5-7.5 to form stable thioether bonds.In aqueous solutions,hydrolytic degradation of the NHS ester is a competing
reaction whose rate increases with pH.The maleimide group is more stable than the NHS-ester group but will slowly
hydrolyze and also lose its reaction specificity for sulfhydryls at pH values>7.5.For these reasons,conjugation
experiments involving these cross-linkers are usually performed at pH 7.2-7.5,with the NHS-ester(amine-targeted)
reaction being accomplished before or simultaneous with the maleimide(sulfhydryl-targeted)reaction.

应用

Sulfo-EMCS is soluble in water and many other aqueous buffers to approximately 10mM,although solubility decreases
with increasing salt concentration.

组份

50mg/bottle

技术规格
 

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