目录号 
            
规格 
            
价格 
            
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C325A
            
10μg
            
400元
            

        
        

            
C325B
            
50μg
            
1000元
        
        

            
C325C
            
1mg
            
8000元
        
    

 
TNFRSF11B, also known as Osteoprotegerin  (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF), is a member of the  tumor necrosis factor receptor superfamily that lacks any apparent cell  association motifs and exists as a soluble secreted protein. Natural  TNFRSF11B/OPG/OCIF has been found to exist predominantly as disulfide  linked dimers. Two TNF superfamily ligands, RANKL (receptor activator of  NFκB ligand) and TRAIL (TNF related apoptosis inducing ligand), have  been shown to be the cellular ligands for TNFRSF11B. TNFRSF11B acts as  decoy receptor for RANKL and thereby neutralizes its function in  osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and  promotes osteoclast apoptosis in vitro. It may also play a role in  preventing arterial calcification. TNFRSF11B may act as decoy receptor  for TRAIL. TRAIL binding blocks the inhibition of osteoclastogenesis.


·Description

Recombinant Human TNFRSF11B produced by  transiently transfected human cell is a secreted protein with sequence  (Accession#O00300)(Glu22-Leu401) of human TNFRSF11B fused with a  polyhistidine tag at the C-terminus.


·Quality Control
Biological activity:

The ED50 of recombinant TNFRSF11B is 8-24ng/mL in the  presence of 12 ng/mL crosslinked rhTRAIL as measured by its ability to  inhibit TRAIL mediated cytotoxicity using L929 mouse fibrosarcoma cells  treated with TRAIL.
Purity: Greater than 90.0% as determined by: 

(a) Analysis by RP-HPLC. 

(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 

Amino-Acid Sequence: Predicted N terminal amino acid is Glu22.

Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHu TNFRSF11B.
 
·Formulation

Sterile and Lyophilized from a 0.2 μm filtered solution in PBS. 



·Reconstitution

It is recommended to reconstitute the lyophilized rHuTNFRSF11B in sterile water not less than 100μg/ml.


·Storage 


Lyophilized rHu MMP3 although stable at room  temperature for 3 weeks, should be stored Lyophilized rHuTNFRSF11B  although stable at room temperature for 3 weeks, should be stored  desiccated below -18°C. Upon reconstitution rHuTNFRSF11B should be  stored at 4°C between 2-7 days and aliquots of reconstituted samples  containing 0.1% BSA are stable up to 3 months below -18°C for future  use.