DCLRE1C polyclonal antibody
产品名称： DCLRE1C polyclonal antibody
英文名称： DCLRE1C polyclonal antibody
产品编号： PAB10023
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Goat polyclonal antibody raised against synthetic peptide of DCLRE1C.
- Immunogen:
- A synthetic peptide corresponding to amino acids 482-495 of human DCLRE1C.
- Host:
- Goat
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:20000
Western Blot (1:500-1:2000)
Immunohistochemistry (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Functional and biochemical dissection of the structure-specific nuclease ARTEMIS.
Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K.EMBO J. 2004 May 5;23(9):1987-97. Epub 2004 Apr 8.
- 2.
- Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families.
Kobayashi N, Agematsu K, Sugita K, Sako M, Nonoyama S, Yachie A, Kumaki S, Tsuchiya S, Ochs HD, Sugita K, Fukushima Y, Komiyama A.Hum Genet. 2003 Apr;112(4):348-52. Epub 2003 Feb 19.
- 3.
- Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow.
Noordzij JG, Verkaik NS, van der Burg M, van Veelen LR, de Bruin-Versteeg S, Wiegant W, Vossen JM, Weemaes CM, de Groot R, Zdzienicka MZ, van Gent DC, van Dongen JJ.Blood. 2003 Feb 15;101(4):1446-52. Epub 2002 Oct 24.
- Applications
- Western Blot (Cell lysate)
- Immunoblotting of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.
CJ is an DCLRE1C deficient cell lineand so no band is visible.
This antibody detects a band of approximately 90 KDa (predicted molecular weight: 78 KDa).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.
Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
- Entrez GeneID:
- 64421
- Protein Accession#:
- Q96SD1;NP_071932
- Gene Name:
- DCLRE1C
- Gene Alias:
- A-SCID,DCLREC1C,FLJ11360,FLJ36438,RS-SCID,SCIDA,SNM1C
- Gene Description:
- DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq
- Other Designations:
- OTTHUMP00000019166,OTTHUMP00000019167,OTTHUMP00000019168,OTTHUMP00000019170,OTTHUMP00000019171,OTTHUMP00000019172,OTTHUMP00000019174,OTTHUMP00000045150,artemis protein,severe combined immunodeficiency, type a (Athabascan)
- Gene Pathway