LGR5 polyclonal antibody
产品名称： LGR5 polyclonal antibody
英文名称： LGR5 polyclonal antibody
产品编号： PAB2591
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of LGR5.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 451-480 at internal region of human LGR5.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of stem cells in small intestine and colon by marker gene Lgr5.
Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H.Nature. 2007 Oct 25;449(7165):1003-7. Epub 2007 Oct 14.
- 2.
- Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors.
McClanahan T, Koseoglu S, Smith K, Grein J, Gustafson E, Black S, Kirschmeier P, Samatar AA.Cancer Biol Ther. 2006 Apr;5(4):419-26. Epub 2006 Apr 19.
- 3.
- Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations.
Yamamoto Y, Sakamoto M, Fujii G, Tsuiji H, Kenetaka K, Asaka M, Hirohashi S.Hepatology. 2003 Mar;37(3):528-33.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of LGR5 polyclonal antibody (Cat # PAB2591) in K-562 cell line lysates (35 ug/lane). LGR5 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5 polyclonal antibody (Cat # PAB2591), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 8549
- Protein Accession#:
- NP_003658;O75473
- Gene Name:
- LGR5
- Gene Alias:
- FEX,GPR49,GPR67,GRP49,HG38,MGC117008
- Gene Description:
- leucine-rich repeat-containing G protein-coupled receptor 5
- Omim ID:
- 606667
- Gene Ontology:
- Hyperlink
- Other Designations:
- G protein-coupled receptor 49,G protein-coupled receptor 67,orphan G protein-coupled receptor HG38
- Gene Pathway