MCM2 monoclonal antibody (M01), clone 6A8
产品名称： MCM2 monoclonal antibody (M01), clone 6A8
英文名称： MCM2 monoclonal antibody (M01), clone 6A8
产品编号： H00004171-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant MCM2.
- Immunogen:
- MCM2 (AAH07670, 805 a.a. ~ 904 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- TQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- MCM2 monoclonal antibody (M01), clone 6A8 Western Blot analysis of MCM2 expression in HeLa NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.
Lane 1: MCM2 transfected lysate(101.9 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 4171
- GeneBank Accession#:
- BC007670
- Protein Accession#:
- AAH07670
- Gene Name:
- MCM2
- Gene Alias:
- BM28,CCNL1,CDCL1,D3S3194,KIAA0030,MGC10606,MITOTIN,cdc19
- Gene Description:
- minichromosome maintenance complex component 2
- Omim ID:
- 116945
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq
- Other Designations:
- DNA replication licensing factor MCM2,MCM2 minichromosome maintenance deficient 2, mitotin,cell devision cycle-like 1,cyclin-like 1,minichromosome maintenance deficient 2 (mitotin),nuclear protein BM28
- Gene Pathway
- Related Disease