Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA Preps
产品名称： Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA Preps
英文名称： Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA Preps
产品编号： 610-06
产品价格： 0
产品产地： invitrogen
品牌商标： invitrogen
更新时间： null
使用范围： null
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Pure, Rapid mRNA Isolation A Straightforward Enrichment Procedure RNA is suitable for all downstream molecular applications including: For Research Use Only. Not for human or animal therapeutic or diagnostic use.
The Dynabeads? mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (Figure 1). Dynabeads? magnetic beads are uniform in size and highly mobile in solution (Figure 2). This enables the bead capture surface to quickly and continuously interact with the entire total RNA sample during the mRNA capture phase. The ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. Only polyadenylated View MoreRNA species (mRNA) are captured resulting in cleaner, more sensitive results (Figure 3). Within about 15 minutes, pure mRNA is isolated and ready for use in downstream applications.
The Dynabeads? mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads?, coupled to oligo-(dT)25, are first equilibrated with Binding Buffer, and then mixed with purified total RNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 μl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.
Gene cloning
cDNA synthesis, cDNA library construction
RT-PCR, Quantitative RT-PCR
RPA (Ribonuclease Protection Assay)
Subtractive Hybridization
Dot/slot Hybridization
Primer extension