AOAC Method 2002.01
a-淀粉酶检测试剂盒
产品名称： a-淀粉酶检测试剂盒
英文名称： α-Amylase SD Assay Kit (High Sensitivity Method)
产品编号： K-AMYLSD
产品价格： 0
产品产地： 爱尔兰
品牌商标： Megazyme
更新时间： null
使用范围： null
上海新睿生物科技有限公司
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K-AMYLSD α-Amylase SD Assay Kit (High Sensitivity Method) 160 / 320 assays (manual) /
640 assays (auto-analyser) ￥4080
淀粉酶SD法是一种高度敏感的比色法，用于测定发芽受损小麦籽粒（也称为收获前发芽或天气受损小麦籽粒）和“晚熟α-淀粉酶”小麦籽粒中的α-淀粉酶。 也可用于测量糖果，软饮料，酿造和发酵，果酱，酱汁，蜜饯，冰淇淋和婴儿食品中的α-淀粉酶。
Validation of Methods
AACC Method 22-02.01
ICC Standard No. 303
Highly sensitive colourimetric method for the determination of
α-Amylase in sprout damaged grain and food products
Principle:
(α-amylase)
(1) Ethylidene-G7-α-PNP + H2O → Ethylidene-GX + G(7-X)-α-PNP
(thermostable α-glucosidase)
(2) G(7-X)-α-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol
Kit size: 160 / 320 assays (manual) / 640 (auto-analyser)
Method: Spectrophotometric at 400 / 405 nm
Total assay time: ~ 5 min
Detection limit: 0.05 U/mL
Application examples: Sprout damaged wheat grain and food products
such as jam sauces conserves and ice cream
Method recognition: AOAC (Method 2002.01) AACC (Method 22-02.01)
and ICC (Standard No. 303)Advantages
- Extremely high sensitivity - 2.4-fold increase over Ceralpha (K-CERA)
- Very cost effective
- All reagents stable for > 2 years after preparation
- Very specific
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for Manual and auto-analyser formats