iScript RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

    Have ease of use and simplicity  one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 510 min
    Benefit from superior compatibility with qPCR directly from cells  sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
    Maximize data from very few cells  reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
    Extend isolated RNA shelf-life  isolated RNA is stable for up to 6 months when frozen
    Use with different cell types  compatible with both suspension and adherent cell cultures