E.coli YK537
产品名称： E.coli YK537
英文名称： E.coli YK537
产品编号： Biovector512353
产品价格： 0
产品产地： Biovector NTCC Inc. USA
品牌商标： Addgene, ATCC, Invi
更新时间： null
使用范围： null
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Product Data Sheet
|
Order ID |
Name |
Description |
|
Biovector206825 |
E.coli YK537 |
E.coli YK537 strain, 300uL. Storage:4℃ |
Description
E. coli YK537 is a mutant strain lacking APase.
Genotype
YK537 (F- leuB6 thi hsdR hsdM lacY rpsL20 galK2 ara-14 xyl-5 Mitl-i supE44 endI- phoA8 recAl)
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain