CDK2/CCNE1 (Human) Recombinant Protein
产品名称： CDK2/CCNE1 (Human) Recombinant Protein
英文名称： CDK2/CCNE1 (Human) Recombinant Protein
产品编号： P4655
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Human CDC2 (NM_001786, 1 a.a. - 298 a.a.) and CCNE1 (M73812, 1 a.a. - 395 a.a. ) recombinanat protein with GST tag expressed in Sf9 cells.
- Theoretical MW (kDa):
- CDK2: 60.222 , CCNE1
- Form:
- Liquid
- Preparation Method:
- Insect cell (Sf9) expression system
- Purification:
- One-step affinity purification using GSH-agarose
- Concentration:
- 0.243 ug/uL
- Activity:
- 241 pmol/ug x min
- Storage Buffer:
- In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 15 mM reduced glutathione, 20% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing
- Quality Control Testing:
- 2 ug/lane SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 1017
- Protein Accession#:
- NM_001798 (Gene ID : 1017);M73812 (Gene ID : 898)
- Gene Name:
- CDK2
- Gene Alias:
- p33(CDK2)
- Gene Description:
- cyclin-dependent kinase 2
- Omim ID:
- 116953
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
- Other Designations:
- cdc2-related protein kinase,cell devision kinase 2,p33 protein kinase
- Entrez GeneID:
- 898
- Protein Accession#:
- NM_001798 (Gene ID : 1017);M73812 (Gene ID : 898)
- Gene Name:
- CCNE1
- Gene Alias:
- CCNE
- Gene Description:
- cyclin E1
- Omim ID:
- 123837
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq
- Other Designations:
- cyclin Es,cyclin Et
