Product Name: Beta-Amyloid (1-42), Ultra Pure, HFIP
Catalog #: P2000022
Amount: 1mg/vial
Storage: -20°
Molecular Mass: 4514.1 4
Peptide Purity: >98%
Counter Ion: HFIP treated
Supplied As: No salt, as HFIP desalts the sample.
Source: Recombinant. A DNA sequence encoding the human beta-amyloid (1-42)sequence was expressed in E. coli.
Sequence:
Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala- Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala
Resuspension:
Resuspend in 1 % NH4OH, at a concentration of 1 mg/ml. Sonicate for 30 seconds to 1 minute after it has gone into solution.
To bring it into your buffer: After resuspension, add 5x or 10x buffer stock and water, to bring to 1x buffer.
Description:
Beta-amyloid peptide (Abeta), the major constituent of amyloid plaques in the brains of Alzheimer’s patients, is thought to be the cause of Alzheimer’s Disease (AD)1-3. AD is the most common neurodegenerative disease and afflicts about 10% of the population over 60 4. The abeta,HFIP is described in Stine, et. al.3.
1) Yankner, BA, et. al., (1990) Science, 250: 279-282
2) Selkoe, D.J., (2001) Physiol. Rev, 81: 741-766
3) Stine, W.B. et. al., (2003) J. Biol. Chem, 278: 11612-11622
4) Frank, R.A., et. al., (2003) Neurobiology of Aging, 24: 521-536
HPLC REPORT:
Product Name: Beta-Amyloid(1-42), HFIP
Column: 4.6*250mm, SP-300-5-C4-BIO
Solvent A: 0.1%Trifluoroacetic in 100% Acetonirile
Solvent B: 0.1%Trifluoroacetic in 100% Water
Gradient: A B
0.01min 10% 90%
25min 100% 0%
25.1min 100% 0%
30min Stop
Flow rate: 1.0ml/min
Wavelength: 220nm
Rank |
Time |
Name |
Conc. |
Area |
1 |
8.000 |
|
0.319 |
4914 |
2 |
9.959 |
|
1.071 |
16490 |
3 |
10.519 |
|
98.23 |
1514485 |
4 |
11.018 |
|
0.2791 |
4298 |
Total |
|
|
100 |
1540187 |
P2000022; Beta Amyloid 1-42
PLlabs其他产品:
β淀粉样肽(1-40),超纯,TFABeta-Amyloid (1-40), Ultra Pure, TFA
β淀粉样肽(1-40),超纯,HFIPBeta-Amyloid (1-40), Ultra Pure, HFIP
β淀粉样肽(1-40),超纯,NaOHBeta-Amyloid (1-40), Ultra Pure, NaOH
β淀粉样肽(1-40),超纯,HClBeta-Amyloid (1-40), Ultra Pure, HCl
β淀粉样肽(1-42),超纯,TFABeta-Amyloid (1-42), Ultra Pure, TFA
β淀粉样肽(1-42),超纯,HFIPBeta-Amyloid (1-42), Ultra Pure, HFIP
β淀粉样肽(1-42),超纯,NaOHBeta-Amyloid (1-42), Ultra Pure, NaOH
β淀粉样肽(1-42),超纯,HClBeta-Amyloid (1-42), Ultra Pure, HC
深圳市华拓生物科技有限公司( www.otwobiotech.com)
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